Linkage study and you can QTL mapping inside the a great tetraploid russet mapping people of potato

0
16

Linkage study and you can QTL mapping inside the a great tetraploid russet mapping people of potato

Genome-wider single nucleotide polymorphism (SNP) indicators along with allele dose pointers possess came up just like the an effective product to have reading state-of-the-art faculties for the grown autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48). At this point, this approach could have been effectively placed on this new identity away from decimal trait loci (QTLs) hidden very heritable faculties such as disease resistance, however, largely unexplored having attributes having advanced models regarding inheritance.

Performance

In this study, an Fstep 1 tetraploid russet mapping population (162 individuals) was evaluated for multiple quantitative traits over two years and two locations to identify QTLs associated with tuber sugar concentration, processing click now quality, vine maturity, and other high-value agronomic traits. We report the linkage maps for the 12 potato chromosomes and the QTL location with corresponding genetic models and candidate SNPs explaining the highest phenotypic variation for tuber quality and maturity related traits. Significant QTLs for tuber glucose concentration and tuber fry color were detected on chromosomes 4, 5, 6, 10, and 11. Collectively, these QTLs explained between 24 and 46% of the total phenotypic variation for tuber glucose and fry color, respectively. The QTL on chromosome 10 was associated with apoplastic invertases, with ‘Premier Russet’ contributing the favorable allele for fry processing quality. On chromosome 5, minor-effect QTLs for tuber glucose concentration and fry color co-localized with various major-effect QTLs, including vine maturity, growth habit, tuber shape, early blight (Altenaria tenuis), and Verticillium wilt (Verticillium spp.).

Results

Linkage studies and you will QTL mapping into the a beneficial russet mapping society (A05141) playing with SNP serving advice effortlessly understood favorable alleles and you may candidate SNPs to own resistance to the fresh accumulation away from tuber reducing sugar. Such novel indicators features a top possibility the improvement off tuber control high quality. Additionally, the fresh new finding of various hereditary designs having traits having overlapping QTLs on readiness locus demonstrably ways another genetic manage.

Records

Which have an intricate polyploid genes the new developed autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48) gifts book demands to own linkage mapping and quantitative trait loci (QTL) data. The present growth of potato unmarried nucleotide polymorphism (SNP) arrays will bring a couple of genome-broad markers which can be used inside the mapping and you may decimal feature locus (QTL) data [step 1,dos,step 3,4]bined on the way to obtain statistical devices that enable use away from allele dosage advice (i.age., what amount of copies of each and every allele from the a given polymorphic locus), it is now possible to help you rather help the energy of SNP recommendations to position recombination occurrences and utilize this short article on the QTL mapping [5,six,7]. Yet, this approach might have been effectively familiar with select novel QTLs and you can applicant SNPs of the late blight opposition [4, 8], interior heat necrosis , Potato malware Y , and you can agronomic qualities , however, largely unexplored to own reading handling quality and other higher-worthy of qualities which have complex heredity.

There clearly was an importance of molecular markers pertaining to tuber handling quality attributes to help your selection of germplasm to own cultivar innovation. Control top quality is basically dependent on tuber glucose blogs. This new buildup out-of fructose and sugar (reducing sugar) for the potato tubers while in the cold storage negatively influences the finish-explore high quality features. Cutting sugar articles is actually a complicated attribute controlled by numerous genetic and you can ecological situations [12, 13]. The brand new cutting-edge genetic buildings on the characteristic is actually consistent with the hobby many enzymes mixed up in metabolic paths connecting starch dysfunction and you may synthesis to sugar creation and you may use [fourteen, 15]. Hereditary mapping studies have shown you to definitely fry handling high quality and you will cutting sugar is of the hundreds of brief feeling QTLs [sixteen,17,18,19,20]. Soltis-Kalina ainsi que al. identified several biggest QTLs for processor chip colour towards chromosomes step 1 and you may six when you look at the an excellent diploid mapping population. Now, Braun ainsi que al. claimed several QTLs getting resistance to cold-caused sweetening to the chromosomes cuatro and you will six out-of diploid potato. Most of these studies have been performed towards the diploid potato germplasm. However, next studies are must finest understand the genetic tissues off such attributes at tetraploid level for the communities derived from russet-skinned potato kinds.

BÌNH LUẬN

Please enter your comment!
Please enter your name here

Website này sử dụng Akismet để hạn chế spam. Tìm hiểu bình luận của bạn được duyệt như thế nào.